Elisa Reader And Washer Principle

The illustration below shows a workflow for a typical sandwich elisa assay.

Elisa reader and washer principle.

What an elisa reader does. An immune reaction i e. And a plate reader software is used to plot standard curves and calculate results. The elisa reader reads the plate.

Are available as fixed as well as adjustable volume as well as single channel and multi channel. The enzyme activity is measured using a substrate that changes color when modified by the enzyme. Along with the enzyme labelling of antigens or antibodies the technique involves following three principles in combination which make it one of the most specific and sensitive than other immunoassays to detect the biological molecule. This ability to wash away non specifically bound materials makes the elisa a powerful tool for measuring specific analytes within a crude preparation.

The test is done in an elisa plate also known as a 96 well plate or microplate. Having the reactants of the elisa immobilized to the microplate surface enables easy separation of bound from non bound material during the assay. A microplate washer is used to wash away non specific material in the wells and an absorbance elisa microplate reader detects the color change produced when target antigen is present. It can be manual system that washes one row or column at a time or semi automated systems that wash one strip or plate at a time or fully automated systems that can process multiple plates.

In short it is an antibody test or a test for immune response to things attacking the body such as virus bacteria and allergens. Readers need to have appropriate filter 650 nm and 450 nm.